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master mix
master mix
Last Update: 2014-11-21
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two microliters of reverse transcribed cdna were then added to the pcr master mix to achieve a final volume of 25 µl.
dois microlitros de cdna transcrito de forma inversa foram então adicionados à mistura principal pcr para chegar ao volume final de 25 µl.
pcr protocols were performed in accordance with the taqman genotyping master mix manufacturer's instructions applied biosystems, ca, usa.
os protocolos de pcr foram feitos de acordo com as instruções do fabricante do taqman® genotyping master mix applied biosystems, ca, eua.
its real time determination was achieved utilizing the sybr® green pcr master mix kit applied biosysystems. thus, the quantity of the pcr product in the reaction is directly proportional to the generation of a fluorescent signal.
sua determinação em tempo real foi realizada utilizando-se o kit sybrÒ green pcr master mix applied biosystems, tendo sido adicionado à reação um intercalante de dna dupla fita, o sybr green i. assim, a quantidade de produto de pcr na reação é diretamente proporcional à geração de um sinal fluorescente.
a master mix containing 12.5 µl sybr green pcr master mix fermentase, germany, 8.5 µl water and 2 µl forward primer and reverse primer in a final volume of 25 µl was prepared to carry out real-time pcr.
uma mistura principal contendo 12,5 µl sybr pcr verde master mix fermentase, germany, 8,5 µl de água e 2 µl de iniciador direto e iniciador reverso num volume final de 25 µl foi preparado para realizar pcr em tempo real.
reactions were performed in 25 µl aliquots containing 12.5 µl sybr green pcr master mix jena bioscience, germany, 1 µl forward primer, 1 µl reverse primer, 8.5 µl rnase- free water, and 2 µl of the cdna.
as reações foram rea lizadas em alíquotas de 25 µl contendo 12,5 µl de sybr green pcr master mix jena bioscience, alemanha, 1 µl de primer direto, 1 µl de primer reverso, 8,5 µl de água rnase-free e 2 µl de dnac.
only master mix solutions which do not contain reagents susceptible to lead to false results due to presence of animal dna shall be used [3].
usar apenas soluções master mix que não contenham reagentes suscetíveis de conduzir a resultados falsos devido à presença de adn animal [3].2.2.2.1.2.3.
identical amounts of cdna and master mix sybrr green qpcr mastermix sabiosciences, qiagen company were distributed to each pcr array well containing portions of specific genes, which had been previously selected.
quantidades idênticas de cdna e do master mix sybr green qpcr mastermix sabiosciences, qiagen company foram distribuídas por cada poço do pcr array contendo as porções dos genes específicos anteriormente escolhidos.
master mix mgcl2 buffer, dntps, and glycerol was combined with 20 u taq polymerase and 1.2 – 3.0 µg dna, and dispensed onto the trays.
o mix master tampão de mgcl2, dntps, e glicerol foi combinado com 20 u taq polimerase e 1,2 – 3,0 µg dna, e dispensado nas placas.
real-time rt-pcr amplification was performed in 20 μl of reaction mixture containing 1μl of cdna sample, 10μl of sybr green realtime pcr master mix toyobo, japan, and 0.25μm of specific primer sets by the use of an abi prism 7900 sequence detection system pe applied biosystems, usa.
a amplificação da pcr-rt em tempo real foi realizada em 20 μl de mistura de reação contendo 1 μl da amostra de cdna, 10 μl de sybr green realtime pcr master mix toyobo, japão, e 0,25 μm de conjuntos de primers específicos mediante a utilização de um sistema de detecção de sequência abi prism 7900 pe applied biosystems, eua.
a total 25 µl of amplification mix consisting of 12.5 µl toptaq dna pcr master mix qiagen - hilden, germany, each primers 1 µl 10ypmol/µl, 8 µl h2o, and 2.5 µl/each sample extraction product was prepared.
um total de 25 µl de mistura de amplificação, consistindo de 12,5 µl de toptaq adn pcr master mix qiagen, hilden, alemanha, 1 µl 10 pmol/µl de cada primer, 8 µl de h2o e 2,5 µl/cada produto da extração da amostra foram preparadas.
==track listing==* united kingdom #1#"why'd you lie to me" (album version) – 3:43#"why'd you lie to me" (m*a*s*h master mix) – 7:03#"bad girls" (live at the brits 2002 with jamiroquai) – 4:13#"why'd you lie to me" (video)* united kingdom #2#"why'd you lie to me" (album version) – 3:43#"why'd you lie to me" (kardinal beats mix) – 4:31#"boom" (album version) – 3:18#"boom" (video)* cassette#"why'd you lie to me" (album version) – 3:43#"why'd you lie to me" (ndb nu soul mix) – 7:18#"why'd you lie to me" (m*a*s*h master mix) – 7:03* france#"why'd you lie to me" (album version) – 3:43#"why'd you lie to me" (m*a*s*h master mix) – 7:03* europe#"why'd you lie to me" (album version) – 3:43#"why'd you lie to me" (m*a*s*h master mix) – 7:03#"why'd you lie to me" (m*a*s*h deep club) – 8:14#"why'd you lie to me" (kardinal beats mix) – 4:31#"why'd you lie to me" (video)* australia#"why'd you lie to me" (album version) – 3:43#"why'd you lie to me" (m*a*s*h deep club) – 8:14#"why'd you lie to me" (kardinal beats mix) – 4:31#"bad girls" (live at the brits 2002 with jamiroquai) – 4:13==charts====references====external links==
==faixas e formatos==* europeu cd 1#"why'd you lie to me" do Álbum 3:45#"why'd you lie to me" master mix 7:03#"why'd you lie to me" deep club 8:14#"why'd you lie to me" beats mix 4:31#"why'd you lie to me" video* europeu cd 2#"why'd you lie to me" do Álbum 3:45#"why'd you lie to me" deep club 8:14#"why'd you lie to me" radio mix#"why'd you lie to me" beats mix 4:31#"bad girls" (com jamiroquai) vivo "the brits" em 2002* europeu promocional single#"why'd you lie to me" do Álbum 3:45* espanhol promocional single#"why'd you lie to me" radio mix#"why'd you lie to me" beats mix 4:31#"why'd you lie to me" do Álbum 3:45* reino unido double cd single setcd1#"why'd you lie to me" do Álbum 3:45#"why'd you lie to me" radio mix#"bad girls" (com jamiroquai) vivo "the brits" em 2002#"why'd you lie to me" videocd2#"why'd you lie to me" do Álbum 3:45#"why'd you lie to me" uk kardinal mix#"boom" do Álbum 3:19#"boom" video* estados unidos edição maxi single#"why'd you lie to me" do Álbum 3:45#"why'd you lie to me" radio mix#"why'd you lie to me" beats mix 4:31#"bad girls" (with jamiroquai) at the brits 2002* reino unido promocional 12" single (m*a*s*h mixes)a-side#"why'd you lie to me" master mix 7:03b-side#"why'd you lie to me" deep club 8:14* reino unido 12" promocional maxi single (kardinal/nu soul mixes)a-side#"why'd you lie to me" beats mix 4:31#"why'd you lie to me" beats instrumental 4:31b-side#"why'd you lie to me" soul dnb mix 6:38==desempenho=====posições===