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fluoreto de fenilmetilsulfonil
benzenemethanesulfonyl fluoride
Last Update: 2014-12-09
Usage Frequency: 4
Quality:
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o fluoreto de fenilmetilsulfonil é uma substância perigosa - manipular com muito cuidado.)
phenylmethylsulphonyl fluoride is harmful - handle with extreme caution.)
Last Update: 2014-11-21
Usage Frequency: 1
Quality:
(nb: o fluoreto de fenilmetilsulfonil é uma substância perigosa - manipular com muito cuidado.)
(nb: phenylmethylsulphonyl fluoride is harmful - handle with extreme caution.)
Last Update: 2014-11-12
Usage Frequency: 2
Quality:
cinco mililitros de uma solução inibitória fluoreto de fenilmetilsulfonil - pmsf 1 mm foram adicionados, bsa a 1% em pbs por 1 g de pellet fecal.
five milliliters of an inhibitory solution phenylmethylsulphonyl fluoride - pmsf 1mm was added, bsa 1% in pbs per 1 g of fecal pellet.
acrescentou-se ao homogeneizado fluoreto de fenilmetilsulfonil na concentração de 100 nm em isopropanol e na quantidade de 10 µl/ml de tampão adicionado, além de um inibidor de proteases.
phenylmethylsulfonyl fluoride was added to the homogenate at a concentration of 100 nm in isopropanol and at a quantity of 10 µl/ml of added buffer, in addition to a protease inhibitor.
deitar fora a fracção sobrenadante e colocar novamente as células em suspensão em aproximadamente 30 ml de pbs que contenha 1 % de sarkosyl e 2 ml de fluoreto de fenilmetilsulfonil (lise).
discard the supernatant and re-suspend the cells in approximately 30 ml of pbs containing 1 % 'sarkosyl' and 2 ml phenylmethylsulphonyl fluoride (lysis buffer).
as amostras do miocárdio foram dissolvidas numa solução tampão contendo tris 10 mm ph 7,5, nacl 150 mm, edta 1 mm, egta 1 mm, naf 50 mm, 0,5 mm fluoreto de fenilmetilsulfonilo, 1 mm de vanadato de sódio, triton x-100 1%, 0,5% de nonidet p-40, e 1μg/ml de leupeptina e aprotinina.
myocardial samples were lysed in a buffer containing 10mm tris ph 7.5, 150mm nacl, 1mm edta, 1mm egta, 50mm naf, 0.5mm phenylmethylsulfonyl fluoride, 1mm sodium vanadate, 1% triton x-100, 0.5% nonidet p-40, and 1μg/ml of leupeptin and aprotinin.