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medir la absorbancia a 256 nm con el espectrofotómetro en comparación con una solución testigo obtenida con 1 ml de solución de hidróxido cálcico (2.1.2) y dilución a 20 ml con agua.
measure the absorbence at 256 nm using the spectrophotometer against a blank consisting of a solution obtained by diluting 1 ml of calcium hydroxide solution (2.1.2) to 20 ml with water.
esperar a que finalice la reacción (unos 5 minutos) y leer las absorbancias de las soluciones testigo y muestra (a2).
wait until the reaction is completed (about five minutes) and read the absorbences of the solutions in the reference and sample cells (a2).