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add 100 μl/well of chromogen/substrate solution (1 ml of abts (2,2'-azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]) 5 mg/ml + 9 ml of substrate buffer (0,1 m phosphate-citrate buffer of ph 4 containing 0,03 % h2o2] and incubate for 10 minutes at room temperature.
100 μl chromogen-/substratlösung [1 ml abts (2,2′-azino-bis-[3-ethylbenzothiazolin-6-sulfonsäure]) 5 mg/ml + 9 ml substrat-puffer (0,1 m citrat-phosphat-puffer mit ph 4 und einem gehalt an 0,03 % h2o2] in jede vertiefung geben und für 10 minuten bei raumtemperatur inkubieren.
Last Update: 2017-04-06
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