DETERMINAZZJONI TA' FLAVOPHOSPHOLIPOL B'DIFFUŻJONI F'MEDJU AGAR

DETERMINATION OF FLAVOPHOSPHOLIPOL BY DIFFUSION IN AN AGAR MEDIUM

Din is-soluzzjoni għandha jkollha kontenut ta' flavophospholipol ta' 0 72 g/ml (= U8).

This solution should have a flavophospholipol content of 0 72 ¶g/ml (= U8).

Iżen kwantità ta' kampjun ta' 5.0 sa 30.0 g li jkun fiha mill-anqas 30 g ta' flavophospholipol.

Weigh out a quantity of samle of 5 70 to 30 70 g containing at least 30 ¶g of flavophospholipol.

Il-metodu huwa għad-determinazzjoni ta' flavophospholipol f'għalf għal bhejjem, konċentrati u taħlitiet lesti minn qabel.

The method is for the determination of flavophospholipol in feedingstuffs, concentrates and premixes.

mg/kg, f'valur assolut, għal kontenut ta' flavophospholipol minn 1 u sa massimu ta' 2 mg/kg;

0 75 mg/kg, in absolute value, for contents of flavophospholipol from 1 and up to 2 mg/kg;

L-attività antibijotika tagħha hija ddeterminata bil-kejl tad-diffużjoni ta' flavophospholipol f'medju agar inokulata bi Staphylococcus aureus.

Its antibiotic activity is determined by measuring the diffusion of flavophospholipol in an agar medium inoculated with Staphylococcus aureus.

Fejn meħtieġ, qabel l-applikar lejn il-kolonna, iddilwixxi l-filtrat bi 80 % metanol (4.6) biex takkwista kontenut mistenni ta' flavophospholipol ta' 16 g/100 ml.

Where necessary, before application to the column, dilute the filtrate with 80 % methanol (4.6) to obtain an expected flavophospholipol content of 16 ¶g/100 ml.

Bl-użu tal-lembut (5.2), ittrasferixxi lejn il-kolonna volum ta' filtrat ta' mill-anqas 100 ml li suppost jkun fih 16 g ta' flavophospholipol (200 ml għal kampjun ta' 30 g ta' għalf tal-bhejjem fi 1 ppm).

Using the funnel (5.2), transfer to the column a volume of filtrate of at least 100 ml which is expected to contain 16 ¶g of flavophospholipol (200 ml for a 30 g sample of feedingstuff at 1 ppm).

Fejn meħtieġ (i.e. meta l-purifikazzjoni tkun ġiet maqbuża), iddilwixxi l-estratt li jirriżulta f'punt 7.1.1 bi 50 % metanol (4.5) biex takkwista l-kontenut kif suppost ta' flavophospholipol ta' 0.2 g/ml (= U8).

Where necessary (i.e. when the purification step has been omitted), dilute the extract obtained in point 7.1.1 with 50 % methanol (4.5) to obtain an expected flavophospholipol content of 0 72 ¶g/ml (= U8).

Bi provi preliminari fuq pjanċi bil-medju ta' l-assaġġ (4.2.2) iddetermina l-kwantità tas-sospensjoni batterjoloġika meħtieġa biex tagħti l-akbar żoni u ż-żoni l-aktar ċari ta' inibizzjoni mal-varji konċentrazzjonijiet ta' flavophospholipol (madwar 30 ml/litru).

By preliminary trials on plates with assay medium (4.2.2) determine the quantity of bacterial suspension required to give the largest and clearest zones of inhibition with the various concentrations of flavophospholipol (about 30 ml/litre).

Stati Membri għandhom jesiġu li l-analiżi għal kontrolli uffiċjali ta' għalf għal bhejjem, f'dak li jirrigwarda l-kontenut fihom ta' baċitraċina taż-żingu, flavophospholipol, ħadid, ramm, manganiż u żingu, li jkunu mwettqa bi qbil mal-metodi kif deskritti fl-Anness ta' din id-Direttiva.

Member States shall require that analyses for official controls of feedingstuffs, as regards their content of zinc bacitracin, flavophospholipol, iron, copper, manganese and zinc, be carried out in accordance with the methods described in the Annex to this Directive.

1. Stati Membri għandhom jesiġu li l-analiżi għal kontrolli uffiċjali ta'għalf għal bhejjem, f’dak li jirrigwarda l-kontenut fihom ta'baċitraċina taż-żingu, flavophospholipol, ħadid, ramm, manganiż u żingu, li jkunu mwettqa bi qbil mal-metodi kif deskritti fl-Anness ta'din id-Direttiva.

1. Member States shall require that analyses for official controls of feedingstuffs, as regards their content of zinc bacitracin, flavophospholipol, iron, copper, manganese and zinc, be carried out in accordance with the methods described in the Annex to this Directive.

Aħsel il-flavophospholipol b'soluzzjoni tal-metanol tal-klorat tal-putassa (4.8) waqt li żżomm ir-rata tat-tnixxija għal madwar 2 ml/minuta.

Elute the flavophospholipol with the methanolic solution of potassium chloride (4.8) keeping the flow rate to about 2 ml/minute.