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An effective approach to recruitment and selection can help an organization to maximize the competitive advantages by choosing the best pool of candidates quickly and cost efficiently (Kleiman, 2005). But successful recruitment and selection can be costly and time-consuming. Still, recruitment and selection is crucial for a business, because unsuccessful recruitment and selection can be responsible for the failure of the business (Ahmed, Tabassum & Hossain, 2006).
Porphyromonas gingivalis infect human through saliva transmission and cause periodontitis. Current diagnosis for periodontitis patients is by clinically identification and confirmation of the bacteria via polymerase chain reaction (PCR), which are costly and time consuming. Therefore the study aims to develop a rapid LAMP assay for detection of P. gingivalis. The P. gingivalis from five saliva samples of patients with periodontal disease and five from healthy patients were detected using two methods, PCR and LAMP assay. Genomic DNA was isolated from saliva sample using two DNA extraction methods, commercial kit and heat-treatment of saliva. The annealing temperatures of 54.2C and isothermal temperature of 65°C was optimized for PCR and LAMP assay respectively. By using PCR, P. gingivalis was detected in 4/5 (100%) of samples using extracted saliva. For LAMP assay P. gingivalis was detected in 4/5 (100%) and 2/5 (40%) of samples using extracted saliva and heat-treated saliva respectively. It conclusion, LAMP assay is more sensitive than PCR for detection of P. gingivalis in saliva samples.
Last Update: 2014-05-05
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