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mcnnw hile prepare the pcr samples. remove the mineral oil (if used) in cach tube wini 50- to 100-pl chloroform. tłie aqueous sample, initially beneath the oil layer, will float on the chloroform-oil mix, which can bc pipeited away. use 10 to 30 jil :or each lane, depending on the size od the well and the dna concentration. add i jil t0x ath:ali denaturmg buffer per 10-pl sample. denature for 10 ruin in a 42’c oven.
mcnnw hile prepare the pcr samples. remove the mineral oil (if used) in cach tube wini 50- to 100-pl chloroform. tłie aqueous sample, initially beneath the oil layer, will float on the chloroform-oil mix, which can bc pipeited away. use 10 to 30 jil :or each lane, depending on the size od the well and the dna concentration. add i jil t0x ath:ali denaturmg buffer per 10-pl sample. denature for 10 ruin in a 42’c oven.