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cloning creates a new, independent copy of the original disk. sharing uses the existing disk image for both the original and the new machine.
pengklonan mencipta salinan cakera asal yang baru dan bebas. perkongsian menggunakan imej cakera sedia ada untuk kedua-dua mesin asal dan yang baru.
you are required to amplify the following gene (sequence is provided below) using pcr for a cloning experiment. design the pcr primers required to amplify the gene.
a) terangkan pertimbangan apabila mereka bentuk primer pcr yang baik untuk pengudaraan pcr rutin
Son Güncelleme: 2021-11-09
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in this study, a flil and a higb deletion mutant was constructed via homologous recombination using pexkm5, a non-replicative plasmid in b. pseudomallei. initially, the upstream fragment (us) and downstream fragment (ds) of the flil and higb gene were amplified and fused together before cloning into the pexkm5 vector.
dalam kajian ini, mutan delesi higb dan flil masing-masing dibina melalui rekombinasi homolog menggunakan pexkm5, plasmid tak bereplika dalam b. pseudomallei. pada mulanya, serpihan huluan (us) dan serpihan hiliran (ds) gen flil dan higb diamplifikasi dan digabungkan sebelum diklonkan ke dalam vektor pexkm5.
Son Güncelleme: 2018-04-07
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