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mir relative quantification was performed by stem loop taqman® rt-qpcr life technologies, ca, usa.
a quantificação relativa de mir foi realizada por stem loop taqman ® rt-qpcr life technologies, ca, eua.
specific stem loop primers were used to synthetize the cdna from 10 ng of total rna using the micro-rna transcription kit life technologies, ca, usa.
primers stem loop específicos foram utilizados para sintetizar o cdna a partir de 10 ng de rna total utilizando o kit de transcrição de micro-rna life technologies, ca, eua.
it has been especially useful in probing the structure of natural rna oligonucleotides, which tend to adopt complex conformations such as stem-loops and pseudoknots.
it has been especially useful in probing the structure of natural rna oligonucleotides, which tend to adopt complex conformations such as stem-loops and pseudoknots.
