Results for ver: translation from English to Persian

ver. alignment
تراز عمودی

ill meet you on the corner of ne and ver .
من تو را توي هر و گز ميبينم .

and i know youre ver i know youre very busy .
و من ميدونم كه تو سرت خيلي شلوغه .

in molecular biology experiments. currently, there are several types of nucleic acid extraction methods with various degrees of complexity. conducting experiments on molecular diagnosis, nucleic acid sequencing, and functional genomics relies on successful nucleic acid extraction. until now, there are two types of kits available for nucleic acid extraction commercially—one is to be used in automatic nucleic acid extractors, which are expensive and large in volume, need to be maintained by trained personnel, and primarily used by large hospitals and national laboratories; the other is more regular, but the process involves centrifugation, is tedious in operation, and depends highly on professional skills, and therefore, is mainly used in research institutions. we developed a multi-functional sample treatment device to fulfil the requirements for nucleic acid extraction. the device was easy to operate with low requirement for trained personnel—without the need for an instrument, a pipette, or a pre-prepared solution, and could be stored at ambient temperatures without cross-contamination. with high extraction purity, the complete process of dna extraction from a sputum sample could be achieved within 15 min. the multi-functional sample treatment tube, designed witha unique perspective, was filled with an optimized lysis solution that could quickly lyse m. tuberculosis cells to release nucleic acids. the lysis solution used in this step could also be used in subsequent nucleic acid amplification reactions, including regular pcr, quantitative fluorescence pcr, psr, and lamp isothermal amplification. the complete extraction process did not involve complicated experimental steps, such as centrifugation, sample introduction, and removal of proteins and other secondary metabolites, and did not include organic solvent extraction or anhydrous ethanol precipitation, thereby saving experimentation time and reducing cross-contamination among samples. the tube had a special design as shown in fig. 1. the tube contained two ends—‘a’ and ‘b’. the end ‘a’ was the heating end where a regular lysis solution was placed, while the end ‘b’ was a sample introduction end where a filtration membrane was fitted on the top that could filter out impurities in the sample or particulates in the lysis solution. moreover, in the middle of the end ‘b’, there was a sample introduction section, which could be opened manually with force by the personnel for squeezing out sample droplets and closed afterwards. optimised lysis solution (1% triton x-100, 1% np-40, 0.1 mm edta, 2% chelex-100): the lysis solution used in our device has been optimised several times, which ensured a certain degree of lysis efficiency of the samples, while not inhibiting the subsequent reactions. when used to treat complex samples (fecal and urine samples), the lysis solution could adsorb impurities from the samples and inhibit the activity of some enzymes to protect the target nucleic acids. simple operational process: as shown in fig. 2, only four simple steps are required to complete the process from a sampling step to a nucleic acid extraction step—open the hinged cover, place a cotton swab containing the sample inside the tube, heat the end ‘a’ for activating the lysis solution, and remove the sample introduction-tip cover in the end ‘b’ to squeeze out sample droplets. the heat lysis was conducted at 95 °c for 10 min. the process was completed within 15 min, providing nucleic acids from the sample to be used for amplification in the downstream process. the multi-functional sample treatment device mentioned above was used to extract nucleic acids from a sputum sample. the extracted nucleic acids were subjected to quantitative fluorescence pcr (capitalbio, china), and the results were then compared to those obtained for the same sputum sample by two commercial kits—takara mini best bacteria genomic dna extraction kit ver.3.0 and qiaamp dna mini kit, as shown in table 1. the data in the table clearly show that our device had nucleic acid extraction efficiency similar to that of the commercially available kits; hence, the device may be used in field settings for rapid nucleic acid extraction from m.
انجام آزمایشاتی در مورد تشخیص مولکولی ، تعیین توالی اسید نوکلئیک و ژنومیک عملکردی بر استخراج موفقیت آمیز اسید نوکلئیک متکی است. تاکنون دو نوع کیت برای استخراج اسید نوکلئیک بصورت تجاری وجود دارد — یکی از آن ها برای استخراج اسید نوکلئیک خودکار استفاده می شود ، که گران و حجم زیادی دارند ، باید توسط پرسنل آموزش دیده نگهداری شوند ، و در درجه اول توسط بیمارستان های بزرگ و آزمایشگاه های ملی؛