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prepare at least two decimal dilutions, viz. and 1/ or more if considered useful, of the resuspended pellet in pelletbuffer.
pripravte minimálne dve desatinné riedenia, t. j. 1/10 a 1/100 alebo viac riedení resuspendovaného peletu v peletovom pufri.
inoculate, by the same technique, 10 seedlings with a suspension of 106 cells per ml from a virulent biovar 2/race 3 strain of ralstonia solanacearum as the positive control and with pelletbuffer as the negative control.
rovnakou technikou naočkujte 10 semenáčov suspenziou virulentného kmeňa ralstoniasolanacearum rasa 3/biovar 2 s hustotou 106 buniek v 1 ml ako pozitívnu kontrolu a peletovým pufrom ako negatívnu kontrolu.