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conditions chromatographigues la phase mobile était constituée de tampon phosphate: 20 mm, 25 mm kci, 1.5 mm mgci 2 ajusté à ph 6.
chromatographic conditions the mobile phase consisted of phosphate buffer: 20 mm, 25 mm kcl, 1.5 mm mgcl 2 adjusted to ph 6.
Last Update: 2014-12-03
Usage Frequency: 1
Quality:
1.2 préparation de la phase stationnaire et des micro-colonnes chromatographigues avant l'immobilisation sur le support chromatographique, les aptamères furent traités en chauffant à 85°c pendant 5 min (tampon phosphate: 25 mm, 25 mm naci, 5 mm mgci 2 ajusté à ph 7.3) et refroidissant à température ambiante pendant ~ 30 ~ mïnutesr 1000 ~ μi d'une suspension de particules de poros streptavidine (20 μm) fournies par la société applied biosystems (courtaboeuf, france) furent mis en contact pendant 3 heures, sous agitation à la température ambiante, avec 60 nmol d'aptamères.
1.2 preparation of the stationary phase and of the chromatographic microcolumns before immobilization on the chromatographic support, the aptamers were treated by heating at 85° c. for 5 min (phosphate buffer: 25 mm, 25 mm nacl, 5 mm mgcl 2 adjusted to ph 7.3) and cooling to ambient temperature for 30 minutes. 1000 μl of a suspension of streptavidin poros particles (20 μm) provided by the company applied biosystems (courtaboeuf, france) were brought into contact for 3 hours, with agitation at ambient temperature, with 60 nmol of aptamers.
Last Update: 2014-12-03
Usage Frequency: 1
Quality: