Results for j'ai us translation from French to English

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j'ai us

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French

la présente invention concerne, l'identification d'un gène codant pour une nouvelle l-arabinose isomérase de la souche bacillus stearothernivphilus us 100 (l,-ai us

English

the invention concerns identification of a gene encoding a novel l-arabinose isomerase of the bacillus stearothernivphilus strain us100 (l-ai us

Last Update: 2011-07-27
Usage Frequency: 1
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French

la présente invention concerne, l'identification d'un gène codant pour une nouvelle l-arabinose isomérase de la souche bacillus stearothernivphilus us 100 (l,-ai us 100), une l-arabinose isomérase exprimée à partir du dit gène, des vecteurs recombinants hébergeant ledit gène, des microorganismes transformés avec ledit vecteur recombinant, un protocole de préparation et de purification de ladite protéine recombinante, une caractérisation biochimique et cinétique de ladite enzyme recombinante et un procédé de bioconversion d'une solution de d-galactose en une solution riche en d-tagatose moyennant ce polypeptide. cette nouvelle protéine possède des caractéristiques originales notamment son indépendance vis-à-vis les ions métalliques pour son activité et sa faible exigence en ces ions pour sa thermostabilité, ainsi que son pouvoir d'isomériser le d-galactose en d-tagatose avec une efficacité importante de l'ordre 48% après 7h à 70°c.

English

the invention concerns identification of a gene encoding a novel l-arabinose isomerase of the bacillus stearothernivphilus strain us100 (l-ai us 100), a l-arabinose isomerase expressed from said gene, recombinant vectors hosting said gene, microorganisms transformed with said gene, a protocol for preparing and purifying said recombinant protein, biochemical and kinetic characterization of said recombinant enzyme and a method for bioconversion of a d-galactose solution into a solution rich in d-tagatose using said polypeptide. this novel protein has original characteristics, in particular its independence from metal ions for its activity and its low need for such ions for its thermostability, as well as its potential for isomerizing d-galactose into d-tagatose with great efficacy of the order of 48 % after 7 hours at 70 °c.

Last Update: 2011-07-27
Usage Frequency: 1
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