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diluire l'antigene btv alla concentrazione pretitolata in pbs, sottoporre a breve sonicazione per disperdere il virus aggregato (in assenza di sonicatore, pipettare vigorosamente) e aggiungere 50 µl in tutti i pozzetti della piastra elisa.
dilute btv antigen to pre-titrated concentration in pbs, sonicate briefly to disperse aggregated virus (if sonicator is not available, pipette vigorously) and add 50 μl to all wells of the elisa plate.
Last Update: 2017-01-31
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