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las secciones se bloquearon con suero de cabra al 5% (<PROTECTED> <PROTECTED>) en <PROTECTED> que contenía albúmina de suero bovino (<PROTECTED>) al 1% y <PROTECTED> x100 (<PROTECTED>t) al 0,1% y se incubaron con anticuerpos <PROTECTED> de conejo purificados por afinidad (<PROTECTED> <PROTECTED>) y con anticuerpos monoclonales <PROTECTED> conjugados con rficoeritrina (<PROTECTED>) (bd <PROTECTED> <PROTECTED>, <PROTECTED> <PROTECTED>, ca) durante 1 h, se lavaron y se tiñeron con anticuerpos de cabra anticonejo conjugados con <PROTECTED> (<PROTECTED> <PROTECTED>) en <PROTECTED>t y en <PROTECTED> al 1% durante 1 h.
sections were blocked with 5% goat serum (<PROTECTED> <PROTECTED>) in <PROTECTED> containing 1% bovine serum albumine (<PROTECTED>) and 0.1% <PROTECTED> x-100 (<PROTECTED>-t), and incubated with rabbit affinity-purified <PROTECTED> antibody (<PROTECTED> <PROTECTED>) and r-phycoerythrin (<PROTECTED>)-conjugated <PROTECTED> monoclonal antibody (bd <PROTECTED> <PROTECTED>, <PROTECTED> <PROTECTED>, ca) for 1 h, washed and stained with <PROTECTED>-conjugated goat anti-rabbit antibody (<PROTECTED> <PROTECTED>) in <PROTECTED>-t and 1% <PROTECTED> for 1 h.