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transdujeron <PROTECTED> y <PROTECTED> de sangre de cordón umbilical humano con lngfrgfp <PROTECTED> lv ex vivo, de acuerdo con los protocolos anteriormente optimizados23, 24 (figura 4).
they transduced human cord-blood <PROTECTED> and <PROTECTED> with lngfr-gfp <PROTECTED> lv ex vivo, according to previously optimized protocols23, 24 (fig.4).
(d, e) se transdujeron linfocitos de sangre periférica humana después de una activación de 2 días con anticuerpos anticd3 y anticd28 (d), o después de 4 días de tratamiento con interleucina7, tal como se describe24, (e), y se analizaron para determinar la expresión de <PROTECTED> y <PROTECTED> tal como se describió anteriormente.
(d, e) human peripheral blood lymphocytes were transduced either after 2-day activation with anti-cd3 and anti-cd28 antibodies (d), or after 4-day treatment with interleukin-7, as described24, (e), and analyzed for <PROTECTED> and <PROTECTED> expression as described above.