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safety and drug tolerability as a prodrug of nucleoside analogue, nhc exerts antiviral effects by causing lethal mutations during the synthesis of viral rna. however, due to the common intermediate of ribose diphosphate shared by rna and dna precursors, nhc possesses the potential toxic risk to the host. the potential host mutagenic activity of nhc was verified by a gene selection system at a concentration of 3 µm in a549 hace2 cells (91). the same results were obtained from the ames experiment