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es una iniciativa del experto independiente de seguros cama chantal marr y seguro lsm. entendemos que la lealtad del cliente se debe ganar y estamos...
is an initiative of independent insurance expert chantal marr bed and lsm insurance. we understand that client loyalty must be earned and we are committed...
colmatador lsm se presenta en envases herméticos, de acuerdo con las directrices de la ce para el envasado, almacenaje y transporte de productos químicos.
laim 2 is packed in hermetic barrels, according to ec packing and storing directives for chemical products. for its storing, maintain the material in its original pack, well closed and in a dry place.
transfección de adn las células fueron alícuotadas en placas de 24 pocillos que contienen cubreobjetos at 104 células / pocillo y se cultivaron durante la noche a aproximadamente el 80% de confluencia. los plásmidos (0,6 mg) fueron transfectadas en células utilizando lipofectamine plustm reactivo (invitrogen / life technologies , reino unido) de acuerdo con las instrucciones del fabricante. cuarenta y ocho horas después de la transfección, las células fueron lavadas dos veces con pbs y luego fixed en un 1,85% de formaldehído (sigma) diluido en pbs que contenía 2% de sacarosa (schwarz / mann, ee.uu.) durante 10 minutos a temperatura ambiente. después de tres lavados con pbs, los cubreobjetos fueron removidos del pocillo y se montaron en af1 (citifluor, reino unido). la visualizacion de plásmidos gfp y rfp las células que expresan gfp-fluorescente mhc i y rfp-e5 se visualizaron, ya sea usando un microscopio leica dmlb o un microscopio zeiss lsm 510 .
dna transfection cells were aliquoted into 24-well plates containing coverslips at 104 cells/well and grown overnight to approximately 80% con¯uence. plasmids (0.6 mg) were transfected into cells using lipofectamine plustm reagent (invitrogen/life tech- nologies, uk) according to the manufacturer's instructions. forty-eight hours after transfection, the cells were washed twice with pbs and then ®xed in 1.85% formaldehyde (sigma) diluted in pbs containing 2% sucrose (schwarz/ mann, usa) for 10 min at room temperature. following a further three washes with pbs, the coverslips were removed from the well and mounted in af1 (citi¯uor, uk). visualization of gfp and rfp plasmids cells expressing ¯uorescent gfp-mhc i and rfp-e5 were visualized either using a leica dmlb microscope or a zeiss lsm 510 microscope.