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mikrobiyal dna'nın doğrudan enfekte dokudan dna ekstraksiyonu: nanogözenek dizilemede kullanım için optimize edilmiş bir protokol
being able to extract microbial dna directly from human samples followed by shotgun metagenomic sequencing, where all dna in a complex sample is identified, can reduce time to diagnosis and targeted treatment. this is especially important in cases with slow-growing or difficult to cultivate microbes. however, in most types of human samples, the proportion of human dna (hdna) to microbial dna is overwhelming and reduces the probability of identifying microbes using ngs technologies. therefore, a dna extraction protocol capable of depleting hdna while simultaneously preserving microbial dna is fundamental to the improvement of ngs microbe detection.