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gute ergebnisse zeigen auch alkalisalze, vorzugsweise natriumsalze, der nitrilotriessigsäure und der ethylendiamintetraessigsäure, sowie tris-hydroxymethylaminomethan.
alkali metals salts, preferably sodium salts, of nitrilotriacetic acid and of ethylenediaminetetraacetic acid, and trishydroxymethylaminomethane, also give good results.
Son Güncelleme: 2014-12-03
Kullanım Sıklığı: 2
Kalite:
um den amilorid-sensitiven natrium-influx zu bestimmen, wurden 100 µl jeder blutprobe in jeweils 5 ml eines hyperosmolaren salz-sucrose-mediums (mmol/l: 140 nacl, 3 kcl, 150 sucrose, 0,1 ouabain, 20 tris-hydroxymethylaminomethan) bei ph 7,4 und 37° c inkubiert. die erythrocyten wurden danach dreimal mit eiskalter mgcl₂-ouabain-lösung (mmol/l: 112 mgcl₂, 0,1 ouabain) gewaschen und in 2,0 ml destilliertem wasser hämolysiert.
to determine the amiloride-sensitive sodium influx, 100 μl of each blood sample were incubated in 5 ml portions each of a hyperosmolar salt/sucrose medium (mmol/l: 140 nacl, 3 kcl, 150 sucrose, 0.1 ouabain, 20 tris-hydroxymethylaminomethane) at ph 7.4 and 37 erythrocytes were then washed three times with ice-cold mgcl.sub.2 /ouabain solution (mmol/l: 112 mgcl.sub.2, 0.1 ouabain) and hemolyzed in 2.0 ml of distilled water.
Son Güncelleme: 2014-12-03
Kullanım Sıklığı: 2
Kalite: