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pipetteer in elk putje 100 μl met hrp geconjugeerd anti-paard gamma-globuline, verdund in pbs + 5 % melk + 0,05 % tween 20, ph 7,2.
dispense 100 μl/well of horseradish-peroxidase (hrp) -conjugated anti-horse gamma-globulin diluted in pbs + 5 % milk + 0,05 % tween 20, ph 7,2.