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4-nitrophenylphosphate phosphohydrolase
4-nitrophenylphosphatase
Last Update: 2014-12-09
Usage Frequency: 4
Quality:
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then the pellet is quickly thawed and resuspended in ice-cold lysing buffer (50 mm hepes ph 7.5, 10 mm mgcl , 1 mm dtt, 5 μg/ml leupeptin, 5 μg/ml aprotinin, 100 μm naf, 100 μm pmsf, 10 mm β-glycerolphosphate, 0.1 mm na vo , 30 mm 4-nitrophenylphosphate) to give 1× cells/17.5 ml.
danach wird das pellet rasch aufgetaut und in eiskaltem lysispuffer (50 mm hepes ph 7,5, 10 mm mgcl , 1 mm dtt, 5 µg/ml leupeptin, 5 µg/ml aprotinin, 100 µm naf, 100 µm pmsf, 10 mm β-glycerolphosphat, 0.1 mm na vo , 30 mm 4-nitrophenylphosphate) zu 1x10 zellen/ 17,5 ml resuspendiert.
Last Update: 2014-12-03
Usage Frequency: 2
Quality: