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reagenti (reakcijski pufer, mgcl2, deoksinukleotidi, začetni oligonukleotidi, termostabilna dna-polimeraza, sterilna voda) se zmešajo skupaj v skladu z razvito metodo, da nastane "izhodiščna zmes".
the reagents (reaction buffer, mgcl2, deoxynucleotides, primers, heat-stable dna-polymerase, sterile water) are mixed together according to the developed method, leading the "mastermix".