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selekcioniranje merilnih epruvet
selection of counter tubes
Last Update: 2014-11-21
Usage Frequency: 1
Quality:
priprava epruvet/ampul
preparation of test tubes/ampoules
Last Update: 2014-10-18
Usage Frequency: 2
Quality:
- 6 steklenih epruvet, 3 z belimi zaporkami in 3 z rdečimi zaporkami,
each pylobactell breath test kit contains: • 1 sachet containing 1 tablet. • 6 glass tubes, 3 with white caps and 3 with red caps. • 30 ml glass mixing tube with cap. • 2 straws. • 1 analysis request form. • 1 security label and 3 additional bar code labels.
z izhlapevanjem pod rahlim tokom dušika odparimo vse topilo tako, da se metilestri koncentrirajo na dnu epruvet.
remove all the solvent by evaporation with a nitrogen stream in such a way that the methyl esters concentrate at the bottom of the tubes.
izmerimo absorbanco raztopin v vsaki od epruvet, v primerjavi s slepo vrednostjo, pri valovni dolžini navedeni v 5.3.
measure the absorbance of the solutions in each of the tubes , compared to the blank value , at the wavelength indicated in 5.3.
veliko število merilnih epruvet, ki bodo potrebne kasneje, se napolni z 10 ml sinteznega etanola brez14c in 10 ml scintilatorja s toluenom.
a larger number of counter tubes than will later be needed are each filled with 10 ml of 14c-free synthesis ethanol and 10 ml toluene scintillator.
v vsako od dveh epruvet (5.4) pipetirajte 1 ml preskusnega vzorca, pri čemer eno epruveto uporabite kot kontrolni vzorec ali negativno kontrolo.
pipette into each of two test tubes (5.4) 1 ml of the test sample, using one tube as a control or blank.
opomba: alternativni postopek z uporabo ekstrakcijskih epruvet s sesalno natego ali nastavki za izpiralko (glej opombo k 5.6) je opisan v dodatku.
note: the alternative procedure using fat-extraction tubes with siphon or wash-bottle fittings (see the note to 5.6.) is described in the appendix.
obarvanost epruvet/ampul s standardno raztopino penicilina (6.5) ostane škrlatno rdeča, da se dokaže zadostna občutljivost preskusnega gojišča.
the coloration of the tubes/ampoules with standard penicillin solution (6.5) shall remain purple to prove the test medium to be sufficiently sensitive.
s pipeto se da v serijo šestih centrifugirnih epruvet (5.6) 0, 1, 2, 3, 4 in 5 ml razredčene standardne raztopine fluorida (4.7.2).
place, by pipette, into a series of six centrifuge tubes (5.6), 0, 1, 2, 3, 4 and 5 ml of the diluted fluoride standard solution (4.7.2).