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a análise imunohistoquímica foi realizada através do método avidina-biotina conjugada a peroxidase goat abc staining system, santa cruz biotechnology inc., santa cruz, ca, eua como descrito previamente por nós em trabalho anterior.brevemente, as lâminas foram desparafinizadas e reidratadas em xilol e alcoóis.
the immunohistochemical analysis was carried out using the peroxidase-conjugated avidin-biotin complex method goat abc staining system, santa cruz biotechnology inc., santa cruz, ca, usa, as described in a previous study conducted by our group.in brief, the slides were deparaffinized and rehydrated in a graded xylene-alcohol series.