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un procédé selon la revendication 5, dans lequel le brai est filé à une force centrifuge relative d'au moins 1000 g .
a process according to claim 5 wherein the pitch is spun at a relative centrifugal force of at least 1000 g.
après incubation, chaque disque de peau est enlevé et jeté et la solution restante est centrifugée pendant 8 minutes à 21 oc (force centrifuge relative ~175 x g).
this rinsing procedure is then repeated, after which the skin discs are removed and placed into vials containing 5 ml of 30 % (w/v) sodium dodecyl sulphate (sds) in distilled water and are incubated overnight at 60 oc. after incubation, each skin disc is removed and discarded and the remaining solution is centrifuged for eight minutes at 21 oc (relative centrifugal force ~175 × g).