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discard the supernatant, drain the tubes thoroughly and resuspend the pellet in pbs by sonication.
supernatanten fjernes, glassene tømmes helt, og massen genopslæmmes i pbs ved hjælp af sonikering.

discard the supernatant, drain the tubes thoroughly and re-suspend the pellet in pbs by sonication.
supernatanten fjernes, glassene tømmes helt, og massen genopslæmmes i pbs ved hjælp af sonikering.

vortex mixing and/or sonication and/or warming to 37 °c may be used, if necessary, to aid solubilisation.
vortexblanding og/eller ultralydbehandling og/eller opvarmning til 37 °c kan anvendes for at øge opløsningsprocessen.

vortex mixing and/or sonication and/or warming to appropriate temperatures may be used to aid solubilisation unless this would affect the stability of the test chemical.
vortexblanding og/eller ultralydbehandling og/eller opvarmning til passende temperaturer kan anvendes for at fremme opløsningen, medmindre dette kan påvirke testkemikaliets stabilitet.

hence, variation of these conditions (co-solvent, trituration, sonication) may be most relevant in the repetition of a test.
en ændring heri (co-solvens, blanding, ultralydbehandling) kan være særdeles relevant ved gentagelsen af en undersøgelse.

to improve the pump performance and to obtain a stable baseline, degas the mobile phase daily by selecting one of the available techniques such as sparging by helium, sonication, vacuum or in-line degassing system.
for at forbedre pumpeevnen og for at opnå en stabil basislinje afgasses den mobile fase dagligt ved anvendelse af en af de disponible metoder, f.eks. gennemstrømning med helium, sonikering, vakuum eller inline-afgasning.