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trypsinized

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Engels

Portugees

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Engels

treated cells were subsequently trypsinized and stored in trizol for the study of rnas and dmso for nuclear extraction at -80ºc.

Portugees

as células tratadas foram subsequentemente tripsinizadas e armazenadas em trizol para o estudo de rnas,e em dmso para extração do núcleo a -80ºc.

Laatste Update: 2020-08-02
Gebruiksfrequentie: 1
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Engels

after growing, the endothelial cells were treated with uremic and healthy media under the same conditions described above, trypsinized and resuspended in 1 ml of medium 199.

Portugees

basicamente, após cultivo as células endoteliais foram tratadas com meio urêmico e meio saudável nas mesmas condições descritas acima, tripsinizadas e ressuspendidas em 1 ml de meio 199.

Laatste Update: 2020-08-01
Gebruiksfrequentie: 1
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Engels

after reaching 90% of confluence one million cells/flask the cells were trypsinized and the fibroblast component was confirmed by flow cytometry, following the protocol of saalbach et al..

Portugees

após chegar a 90% de confluência um milhão de células/frasco, as células foram tripsinizadas e o componente fibroblasto foi confirmado por meio de citometria, seguindo o protocolo de saalbach et al..

Laatste Update: 2020-08-02
Gebruiksfrequentie: 1
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Engels

in order to read viability only 10µl trypsinized cell suspension were mixed with 0.3µl of the solution were used of acridine orange dyes and ethidium bromide 100 µg/ml, in a ratio v: v 1:1.

Portugees

para a leitura da viabilidade foram utilizados apenas 10ml da suspensão de células tripsinizadas que foram misturadas a 0,3ml da solução dos corantes acridineorange e brometo de etídio 100mg/ml, numa relação v:v 1:1.

Laatste Update: 2020-08-01
Gebruiksfrequentie: 1
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