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stock culture
založna kultura
Last Update: 2014-11-21
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three strains, wp2, wp2 uvr a and wp2 uvr a pkm 101 should be used. recognized methods of stock culture preparations and storage are to be used.
uporabiti je treba tri seve: wp2, wp2 uvr a in wp2 uvr a pkm 101. uporabiti je treba priznane metode priprave in shranjevanja založnih kultur.
cells from frozen stock cultures are seeded in culture medium at an appropriate density and subcultured at least once before they are used in the in vitro 3t3 nru phototoxicity test.
celice iz zamrznjenih založnih kultur se v umetno gojišče zasejejo v ustrezni gostoti in se ponovno kultivirajo vsaj enkrat, preden se uporabijo v preskusu fototoksičnosti 3t3 nru in vitro.
cells are propagated from stock cultures, seeded in culture medium at a density such that the cultures will not reach confluency before the time of harvest, and incubated at 37 °c.
celice se namnožijo iz založnih kultur, nasadijo v gojišče tako na gosto, da kultura ne bo dosegla konfluentne rasti, preden se požanjejo, in se inkubirajo pri 37 °c.
the stock cultures are small algal cultures that are regularly transferred to fresh medium to act as initial test material.
založne kulture so majhne kulture alg, ki se redno prenašajo v sveža gojišča in se uporabljajo kot začetni preskusni material.
the spore suspension must be uniformly turbid and if it contains flocks or sediment it should be discarded and a new suspension should be prepared from the stock culture (5.4.2).
suspenzija spor mora biti enakomerno motna, če pa vsebuje kosmiče ali usedlino, bi jo bilo treba zavreči in iz matične kulture (5.4.2) pripraviti novo suspenzijo.