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očitna neporavnanost
obvious misalignment
Last Update: 2017-04-06
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strukturna poškodba kromosoma, izražena kot prelom posameznih kromatid ali prelom in združitev med kromatidama.
structural chromosome damage expressed as breakage of single chromatids or breakage and reunion between chromatids.
pri soležnih zvarih neporavnanost spojnih prerezov ne sme presegati petine debeline sten (1/5 a).
in the case of butt welds, the misalignment of the joint faces may not exceed one-fifth of the thickness of the walls (1/5 a).
za vsako obdelano in kontrolno kulturo posebej je treba navesti število izmenjav sestrskih kromatid za vsako metafazo in število sestrskih kromatid na kromosom za vsako metafazo.
the number of sces for each metaphase and the number of sces per chromosome for each metaphase should be listed separately for all treated and control cultures.
presledek: akromatska lezija, manjša od širine ene kromatide, ob zelo majhni neporavnanosti kromatid(-e).
gap: an achromatic lesion smaller than the width of one chromatid, and with minimum misalignment of the chromatid(s).
končna koncentracija nosilca v gojitvenem sistemu ne sme bistveno vplivati na sposobnost preživetja celic ali stopnjo rasti, vplive na frekvenco izmenjav sestrskih kromatid pa je treba spremljati z uporabo kontrole s topilom.
the final concentration of a vehicle in the culture system should not significantly affect, cell viability or growth rate and effects on sce frequency should be monitored by a solvent control,
obarvanje stekelc, za prikaz izmenjav sestrskih kromatid, je mogoče opraviti z več tehnikami (npr. fluorescenca plus giemsa metoda).
staining of slides to show sces can be performed by several techniques, (e.g. the fluorescence plus giemsa method).
testi in vivo (mikronukleusni test, test izmenjave sestrskih kromatid, test nepredvidene sinteze dna, dominantni letalni test) niso pokazali genotoksičnega potenciala.
in vivo tests (micronucleus, sister chromatid exchange, unscheduled dna synthesis, dominant lethal tests) did not show any genotoxic potential.