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kelman’s tetrazolium medium
kelmana tetrazola barotne
Last Update: 2014-11-21
Usage Frequency: 1
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smsa selective medium (engelbrecht, 1994 as modified by elphinstone et al, 1996) but delete the agar and the tetrazolium salts.
smsa selektīvā barotne (engelbrecht, 1994 atbilstoši elphinstone et al., 1996grozījumiem), svītrojot agaru un tetrazola sāļus.
add a filter-sterilised aqueous solution of triphenyl tetrazolium chloride (sigma) to obtain a final concentration of 50 mg per litre.
pievienot filtrējot sterilizētu trifeniltetrazola hlorīda (sigma) šķīdumu ūdenī, iegūstot galīgo koncentrāciju 50 mg litrā.
on kelman's tetrazolium medium, typical colonies of virulent isolates of ralstonia solanacearum are cream, flat, irregular and fluidal with blood red coloured whorls in the centre.
kelmana tetrazola barotnē ralstonia solanacearum virulento izolātu tipiskās kolonijas ir krēmkrāsas, plakanas, neregulāras un ar šķidru konsistenci, to centrā veidojas asinssarkani spirāles veida vijumi.
cool to 50 °c and add a filter-sterilised solution of 2,3,5-triphenyl tetrazolium chloride (sigma) to obtain a final concentration of 50 mg per l.
atdzesēt līdz 50 °c un pievienot ar filtrāciju sterilizētu 2,3,5-trifeniltetrazola hlorīda (sigma) šķīdumu, iegūstot galīgo koncentrāciju 50 mg/l.