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skölj ur 40-60 odlingsflaskor med sammanhängande cellager av bhk-21-celler tre gånger med serumfritt eagles medium och infektera med bluetonguevirus serotyp 1 i serumfritt eagles medium.
wash 40-60 roux of confluent bhk-21 cells three times with serum-free eagle's medium and infect with bluetongue virus serotype 1 in serum-free eagle's medium.
Last Update: 2014-11-21
Usage Frequency: 1
Quality:
samla in viruset när cpe är fullständig i 90 % till 100 % av cellagret i varje odlingsflaska genom att skaka av alla celler som fortfarande sitter kvar på glaset.
when cpe are complete in 90 to 100 % of the cell sheet of each roux, harvest the virus by shaking any still-attached cells from the glass.
Last Update: 2014-11-21
Usage Frequency: 3
Quality: